6UHY
WDR5 in complex with Myc site fragment inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.464, 46.955, 112.770 |
Unit cell angles | 90.00, 117.63, 90.00 |
Refinement procedure
Resolution | 31.297 - 1.260 |
R-factor | 0.1554 |
Rwork | 0.154 |
R-free | 0.18140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.855 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.280 |
High resolution limit [Å] | 1.260 | 1.260 |
Rpim | 0.035 | |
Number of reflections | 162176 | 7904 |
<I/σ(I)> | 33.4 | |
Completeness [%] | 96.2 | |
Redundancy | 4.1 | |
CC(1/2) | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Bis-Tris pH 6.0, 0.2 M ammonium acetate, 28% to 32% PEG3350. |