6UEU
Crystal structure of BF DNA polymerase F710Y mutant bound to tetrahydrofuran and dATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.875, 109.068, 150.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.987 - 1.800 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hvi |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.987 | 44.987 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.340 | 1.800 |
| Rmerge | 0.078 | 0.037 | 0.595 |
| Rmeas | 0.089 | 0.041 | 0.678 |
| Total number of observations | 632948 | ||
| Number of reflections | 138448 | 5091 | 21082 |
| <I/σ(I)> | 12.27 | 35.88 | 2.79 |
| Completeness [%] | 96.9 | 87.3 | 99.7 |
| Redundancy | 4.572 | 4.787 | 4.468 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 50% (v/v) saturated ammonium sulfate, 20 mM MgSO4, 100 mM MES, 1% MPD |
