6UEK
Structure of Urocanate Hydratase from Trypanosoma cruzi in complex with NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.614, 134.788, 113.399 |
| Unit cell angles | 90.00, 93.25, 90.00 |
Refinement procedure
| Resolution | 113.220 - 2.160 |
| Rwork | 0.218 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uwk |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.220 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Number of reflections | 128119 | 9011 |
| <I/σ(I)> | 15.52 | 2.05 |
| Completeness [%] | 94.3 | 84.2 |
| Redundancy | 5.7 | 4.1 |
| CC(1/2) | 0.998 | 0.682 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 8000, potassium dihydrogen phosphate, glycerol |
