6U43
Crystal structure of Methanoperedens nitroreducens elongation factor 2 H595N bound to GMPPCP and magnesium (triclinic crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 |
Unit cell lengths | 57.195, 62.524, 63.396 |
Unit cell angles | 64.21, 69.68, 80.51 |
Refinement procedure
Resolution | 54.264 - 1.400 |
R-factor | 0.1665 |
Rwork | 0.165 |
R-free | 0.19160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Saccharomyces cerevisiae EF-2 (PDB entry 1N0V) |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.300 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.044 | 0.545 |
Number of reflections | 131884 | 17221 |
<I/σ(I)> | 9.7 | 1.2 |
Completeness [%] | 90.3 | |
Redundancy | 2.2 | |
CC(1/2) | 0.999 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100 mM HEPES, pH 7.1-7.6, 200 mM ammonium sulfate, 5-15 % (v/v) isopropanol, 16.5-20 % (w/v) polyethylene glycol (PEG) 4000, 9 mM GppCp, and 15 mM MgCl2 |