6U3W

Crystal structure of yeast alpha/epsilon-COP of the COPI vesicular coat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9782
Spacegroup name	P 21 21 21
Unit cell lengths	72.264, 84.594, 117.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.460 - 2.394
R-factor	0.1824
Rwork	0.178
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mv2
RMSD bond length	0.007
RMSD bond angle	0.884
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.440
High resolution limit [Å]	2.390	6.500	2.390
Rmerge	0.104	0.054	0.673
Rmeas	0.114	0.060	0.767
Rpim	0.047	0.025	0.360
Number of reflections	29047	1591	1404
<I/σ(I)>	5.1
Completeness [%]	99.7	99.7	98.1
Redundancy	5.6	5.2	4.4
CC(1/2)		0.996	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1 M Tris, pH 8.5, 0.2 M Li2SO4, 15% (w/v) PEG 3350, 25% (v/v) glycerol Cryoprotected with 23% (w/v) PEG 3350, 25% (v/v) glycerol