6U3A

1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979460
Spacegroup name	P 21 21 21
Unit cell lengths	63.419, 68.077, 86.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.661 - 1.650
R-factor	0.1795
Rwork	0.178
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2be6
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	35.661	1.750
High resolution limit [Å]	1.650	4.930	1.650
Number of reflections	85584	3173	13835
<I/σ(I)>	13.5
Completeness [%]	98.6
Redundancy	2.93
CC(1/2)	0.999	0.999	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	DL-malic acid