6U2U
Helix-Loop-helix motif of mouse DNA-binding protein inhibitor ID-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.893, 57.712, 32.097 |
| Unit cell angles | 90.00, 114.29, 90.00 |
Refinement procedure
| Resolution | 29.256 - 1.500 |
| R-factor | 0.2059 |
| Rwork | 0.202 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mgm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.803 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.234 | |
| Rpim | 0.028 | |
| Number of reflections | 13599 | 1182 |
| <I/σ(I)> | 35.9 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 299 | 0.1 M MES (pH 6.5), 0.2 M sodium acetate |
