6U11

Xenopus laevis N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) (C46S C219S C453S C480S C486S) with CTD mostly flexible

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	67.019, 383.969, 96.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.450 - 2.700
R-factor	0.1985
Rwork	0.196
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pkg
RMSD bond length	0.003
RMSD bond angle	0.569
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.450	2.800
High resolution limit [Å]	2.700	2.700
Rmeas	0.204
Number of reflections	36872	2430
<I/σ(I)>	5.92	1
Completeness [%]	71.5
Redundancy	2.9
CC(1/2)	0.970	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	potassium formate, tris pH 8