6U11

Xenopus laevis N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) (C46S C219S C453S C480S C486S) with CTD mostly flexible

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	EGF-like domain-containing protein	polymer	497	54264.1	1	UniProt (A0A1L8HDP6) Pfam (PF09992) In PDB	Xenopus laevis (African clawed frog)
2	B	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
3	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	A	POTASSIUM ION	non-polymer		39.1	11	Chemie (K)
6		water	water		18.0	141	Chemie (HOH)

Sequence modifications

A: 17 - 503 (UniProt: A0A1L8HDP6)

PDB	External Database	Details
Asp 7	-	expression tag
Arg 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Ser 46	Cys 96	engineered mutation
Met 141	Ile 191	engineered mutation
Ser 219	Cys 269	engineered mutation
Ile 251	Leu 301	engineered mutation
Ser 453	Cys 503	engineered mutation
Ser 480	Cys 530	engineered mutation
Ser 486	Cys 536	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54264.1
Branched	Number of molecules	2
	Total formula weight	994.9
Non-Polymers*	Number of molecules	12
	Total formula weight	651.3
All*	Total formula weight	55910.4

*Water molecules are not included.