6TZT

Crystal structure of human alpha/epsilon-COP of the COPI vesicular coat bound to alpha-COP STM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 31 2 1
Unit cell lengths	138.470, 138.470, 192.868
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.624 - 3.065
R-factor	0.18
Rwork	0.178
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mkr
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.624	29.620	3.140
High resolution limit [Å]	3.065	13.710	3.065
Rmerge	0.090	0.035	0.795
Rmeas	0.094	0.037	0.839
Rpim	0.029	0.012	0.265
Total number of observations		4387	28290
Number of reflections	40664	471	2924
<I/σ(I)>	20.6	57.6	2.8
Completeness [%]	99.7	90.5	97.9
Redundancy	10.2	9.3	9.7
CC(1/2)	0.999	0.999	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	300	0.1 M sodium citrate, pH 6.0, 1% (v/v) Tacsimate, pH 5.0, 9% (w/v) PEG 3350, 5 mM dithiothreitol Cryoprotected with 30% (v/v) ethylene glycol.