6TYH
Four-Disulfide Insulin Analog A22/B22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.231, 61.821, 60.468 |
| Unit cell angles | 90.00, 112.81, 90.00 |
Refinement procedure
| Resolution | 35.595 - 1.600 |
| R-factor | 0.165802433142 |
| Rwork | 0.162 |
| R-free | 0.19579 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rpim | 0.263 | |
| Number of reflections | 81415 | 4121 |
| <I/σ(I)> | 20.8 | 2.3 |
| Completeness [%] | 98.5 | 97.01 |
| Redundancy | 13.2 | |
| CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium Citrate, Phenol, Acetone, Zinc acetate |
