6TYF

Crystal structure of MTB sigma L transcription initiation complex with 6 nt long RNA primer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	126.431, 161.781, 213.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.260 - 3.800
R-factor	0.2577
Rwork	0.256
R-free	0.29990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.748
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.260	3.910
High resolution limit [Å]	3.780	3.780
Number of reflections	42516	3902
<I/σ(I)>	5.6
Completeness [%]	95.0
Redundancy	8.3
CC(1/2)	0.622	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM sodium citrate tribasic dihydrate, pH 5.6, 200 mM sodium acetate, and 10% PEG4000