6TYB
Isolation and Structure of an Antibody that Fully Neutralizes Isolate SIVmac239 Reveals Functional Similarity of SIV and HIV Glycan Shields
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 197.143, 197.143, 178.531 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.867 - 2.301 |
R-factor | 0.1796 |
Rwork | 0.178 |
R-free | 0.20970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cay |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_3584) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.105 | 0.053 | 0.708 |
Rmeas | 0.111 | 0.055 | 0.917 |
Rpim | 0.034 | 0.017 | 0.573 |
Number of reflections | 84058 | 4890 | 2028 |
<I/σ(I)> | 10.7 | ||
Completeness [%] | 92.7 | 99.4 | 45.3 |
Redundancy | 8.8 | 10.2 | 1.5 |
CC(1/2) | 0.998 | 0.464 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 6% PEG 8000, 17% isopropanol, 0.1M imidazole pH 6.5. Crystals were flash frozen in liquid nitrogen with 20 % (v/v) ethylene glycol as a cryoprotectant. |