6TXQ
The high resolution structure of the FERM domain and helical linker of human moesin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 112.419, 112.419, 61.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.492 - 1.730 |
| Rwork | 0.200 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e5w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.560 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.420 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.260 | 16.039 |
| Rmeas | 0.262 | 16.345 |
| Rpim | 0.038 | 3.121 |
| Number of reflections | 41463 | 2210 |
| <I/σ(I)> | 15.6 | 0.7 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 88.2 | 51.5 |
| CC(1/2) | 0.999 | 0.377 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M ammonium acetate, 0.1M tris pH 8.5, 32% propan-2-ol |
