6TXB

Crystal structure of Mindy1 mutant (P138A) in complex with Lys48 linked di-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99187
Spacegroup name	P 41 2 2
Unit cell lengths	74.032, 74.032, 202.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.840 - 2.180
R-factor	0.2142
Rwork	0.212
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jkn 1ubq
RMSD bond length	0.006
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.840	2.250
High resolution limit [Å]	2.180	2.180
Rmerge	0.071	0.987
Rmeas	0.077	1.066
Rpim	0.029	0.397
Number of reflections	30435	2560
<I/σ(I)>	14.2
Completeness [%]	100.0	100
Redundancy	7	7.1
CC(1/2)	0.999	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.2 M Sodium malonate, 20% PEG 3350