6TVV

Crystal structure of 3'-5' RecJ exonuclease from M. Jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.89113
Spacegroup name	P 41 21 2
Unit cell lengths	92.070, 92.070, 275.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.330 - 2.800
R-factor	0.21683
Rwork	0.214
R-free	0.26389
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.601
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.900	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.082
Number of reflections	30267	4308
<I/σ(I)>	13.7	4.4
Completeness [%]	99.2
Redundancy	6.9
CC(1/2)		0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Sodium citrate, 0.1 M Bis-Tris-Propane pH 8.5, 14-24 % w/v PEG 3350, 10 mM MnCl2