6TUG
Enterococcus italicus Csm6 bound to cyclic hexa-2'-fluoro-hexa-dAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 93.604, 106.686, 116.793 |
| Unit cell angles | 90.03, 90.01, 90.10 |
Refinement procedure
| Resolution | 48.510 - 2.420 |
| R-factor | 0.2648 |
| Rwork | 0.264 |
| R-free | 0.29170 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.786 |
| Data reduction software | XDS |
| Data scaling software | autoPROC (1.0.5) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.510 | 2.510 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.076 | 0.473 |
| Rmeas | 0.090 | 0.554 |
| Rpim | 0.047 | 0.285 |
| Number of reflections | 156394 | 16789 |
| <I/σ(I)> | 13.12 | |
| Completeness [%] | 90.8 | 97.78 |
| Redundancy | 3.5 | 3.7 |
| CC(1/2) | 0.998 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM HEPES pH 7.5 1.6 M Ammonium sulphate 25 mM Lithium perchlorate |
