6TTX
Crystal structure of the human METTL3-METTL14 complex bound to Compound 5 (ASI_M3M_051)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-18 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.210, 64.210, 226.380 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.770 - 2.000 |
R-factor | 0.1927 |
Rwork | 0.191 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l6d |
RMSD bond length | 0.009 |
RMSD bond angle | 0.949 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.270 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 37538 | 5913 |
<I/σ(I)> | 13.35 | |
Completeness [%] | 99.8 | |
Redundancy | 6.5 | |
CC(1/2) | 0.999 | 0.674 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 200 mM Magnesium acetate |