Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6TTX

Crystal structure of the human METTL3-METTL14 complex bound to Compound 5 (ASI_M3M_051)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0001510	biological_process	RNA methylation
A	0001734	molecular_function	mRNA m(6)A methyltransferase activity
A	0002376	biological_process	immune system process
A	0003723	molecular_function	RNA binding
A	0003729	molecular_function	mRNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006397	biological_process	mRNA processing
A	0006974	biological_process	DNA damage response
A	0007283	biological_process	spermatogenesis
A	0007623	biological_process	circadian rhythm
A	0008168	molecular_function	methyltransferase activity
A	0008173	molecular_function	RNA methyltransferase activity
A	0009048	biological_process	dosage compensation by inactivation of X chromosome
A	0016556	biological_process	mRNA modification
A	0016604	cellular_component	nuclear body
A	0016607	cellular_component	nuclear speck
A	0016740	molecular_function	transferase activity
A	0019827	biological_process	stem cell population maintenance
A	0021861	biological_process	forebrain radial glial cell differentiation
A	0030154	biological_process	cell differentiation
A	0031053	biological_process	primary miRNA processing
A	0032259	biological_process	methylation
A	0034644	biological_process	cellular response to UV
A	0036396	cellular_component	RNA N6-methyladenosine methyltransferase complex
A	0042063	biological_process	gliogenesis
A	0043489	biological_process	RNA stabilization
A	0045087	biological_process	innate immune response
A	0045580	biological_process	regulation of T cell differentiation
A	0045727	biological_process	positive regulation of translation
A	0045746	biological_process	negative regulation of Notch signaling pathway
A	0046982	molecular_function	protein heterodimerization activity
A	0048477	biological_process	oogenesis
A	0048511	biological_process	rhythmic process
A	0051445	biological_process	regulation of meiotic cell cycle
A	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
A	0061157	biological_process	mRNA destabilization
A	0098508	biological_process	endothelial to hematopoietic transition
A	1902036	biological_process	regulation of hematopoietic stem cell differentiation
A	1903679	biological_process	positive regulation of cap-independent translational initiation
A	1904047	molecular_function	S-adenosyl-L-methionine binding
A	1990931	molecular_function	mRNA N6-methyladenosine dioxygenase activity
B	0000398	biological_process	mRNA splicing, via spliceosome
B	0001510	biological_process	RNA methylation
B	0001734	molecular_function	mRNA m(6)A methyltransferase activity
B	0003723	molecular_function	RNA binding
B	0003729	molecular_function	mRNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0006397	biological_process	mRNA processing
B	0006974	biological_process	DNA damage response
B	0007283	biological_process	spermatogenesis
B	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
B	0016556	biological_process	mRNA modification
B	0019827	biological_process	stem cell population maintenance
B	0021861	biological_process	forebrain radial glial cell differentiation
B	0030154	biological_process	cell differentiation
B	0031053	biological_process	primary miRNA processing
B	0031667	biological_process	response to nutrient levels
B	0036396	cellular_component	RNA N6-methyladenosine methyltransferase complex
B	0042063	biological_process	gliogenesis
B	0045580	biological_process	regulation of T cell differentiation
B	0045664	biological_process	regulation of neuron differentiation
B	0045727	biological_process	positive regulation of translation
B	0048255	biological_process	mRNA stabilization
B	0061157	biological_process	mRNA destabilization
B	0140640	molecular_function	catalytic activity, acting on a nucleic acid
B	1901533	biological_process	negative regulation of hematopoietic progenitor cell differentiation
B	1904047	molecular_function	S-adenosyl-L-methionine binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	binding site for residue NX8 A 601

Chain	Residue
A	CYS376
A	GLY548
A	ASN549
A	GLN550
A	HOH719
A	HOH747
A	HOH775
A	ASP377
A	ILE378
A	ASP395
A	PRO397
A	LEU409
A	SER511
A	PHE534
A	ARG536

site_id	AC2
Number of Residues	5
Details	binding site for residue ACT B 501

Chain	Residue
A	GLU438
B	ARG249
B	ARG254
B	ARG255
B	HOH616

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	Region: {"description":"Interaction with METTL14","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Region: {"description":"Gate loop 2","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27373337","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27627798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7W","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5L6D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5L6E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27373337","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7U","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5K7W","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	2
Details	Site: {"description":"Interaction with METTL14","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	14
Details	Region: {"description":"Interaction with METTL3","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	9
Details	Region: {"description":"Positively charged region required for RNA-binding","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	2
Details	Site: {"description":"Interaction with METTL3","evidences":[{"source":"PubMed","id":"27281194","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5IL0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5IL2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)