6TTK

Crystal structure of the kelch domain of human KLHL12 in complex with DVL1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	298
Detector technology	PIXEL
Collection date	2017-09-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	80.225, 73.145, 101.845
Unit cell angles	90.00, 94.50, 90.00

Refinement procedure

Resolution	79.978 - 2.383
R-factor	0.2266
Rwork	0.225
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vpj
Data reduction software	xia2
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.980	79.980	2.510
High resolution limit [Å]	2.380	7.540	2.380
Rmerge	0.204	0.095	0.741
Rmeas	0.232	0.108	0.840
Rpim	0.107	0.051	0.388
Total number of observations	212254	6803	31109
Number of reflections	47109	1562	6860
<I/σ(I)>	5.8	10.2	2.2
Completeness [%]	99.8	99.3	99.7
Redundancy	4.5	4.4	4.5
CC(1/2)	0.984	0.989	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	30% PEG4000, 0.2 M ammonium acetate, 0.1 M acetate pH 4.6