6TM7
Human 14-3-3 sigma isoform in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.270, 102.660, 157.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.650 - 3.000 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yz5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.788 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.970 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.198 | 0.608 |
| Number of reflections | 21085 | 3016 |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 40 % wt/vol PEG 4000, 600mM ammonium sulfate, 100 mM Tris-HCl, pH 9.0 |
