6TM1

Crystal structure of the DHR2 domain of DOCK10 in complex with RAC3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.980
Spacegroup name	I 2 2 2
Unit cell lengths	110.079, 128.455, 215.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 3.710
R-factor	0.2624
Rwork	0.260
R-free	0.32110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wm9 2c2h
RMSD bond length	0.004
RMSD bond angle	0.823
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	3.850
High resolution limit [Å]	3.710	3.710
Rmerge	0.080	0.300
Number of reflections	29674	1515
<I/σ(I)>	6.61
Completeness [%]	95.5
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	25% (w/v) PEG 1500, 100 mM MMT. MMT buffer is produced by mixing DL-malic acid, MES and Tris base in the molar ratios of 1:2:2.