6TKV
Crystal structure of the human FUT8 in complex with GDP and a biantennary complex N-glycan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-24 |
Detector | DECTRIS PILATUS3 X 1M |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.039, 68.589, 173.921 |
Unit cell angles | 90.00, 149.90, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.1868 |
Rwork | 0.185 |
R-free | 0.21884 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2de0 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.065 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.010 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.133 | 1.368 |
Number of reflections | 89000 | 12914 |
<I/σ(I)> | 6.4 | 1.4 |
Completeness [%] | 99.9 | |
Redundancy | 4.4 | |
CC(1/2) | 0.995 | 0.447 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Carboxilic acids 0.1 M Buffer system 3 pH 8.5 30% Precipitant mix 1 (Molecular Dimensions). |