6TIY
DROSOPHILA GMPCPP-TUBULIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.669, 126.564, 251.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.293 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.21710 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ryc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.210 | 2.350 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 95769 | 6535 |
| <I/σ(I)> | 13.71 | 1.23 |
| Completeness [%] | 99.4 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.999 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG1500, LISO4, PIPES BUFFER, PH 6.8 |
