6THG

Cedar Virus attachment glycoprotein (G) in complex with human ephrin-B1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	112.796, 138.435, 235.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.218 - 4.074
R-factor	0.2778
Rwork	0.276
R-free	0.31410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6thb
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.170	4.140
High resolution limit [Å]	4.070	4.070
Rmerge	0.407	2.192
Rpim	0.173	0.914
Number of reflections	30120	1373
<I/σ(I)>	3.4	0.9
Completeness [%]	99.8	92.4
Redundancy	6.5
CC(1/2)	0.965	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% polyethylene glycol 1500 (w/v), 0.1 M PCB (sodium propionate, sodium cacodylate, and BIS-TRIS propane) pH 5.0, and 0.02 M cadmium chloride dihydrate.