6TGE

NADP dependent methylene-tetrahydromethanopterin dehydrogenase-NADP+-methenyl-H4MPT+ complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-11-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	98.640, 123.500, 167.300
Unit cell angles	94.79, 100.39, 108.97

Refinement procedure

Resolution	48.761 - 1.500
R-factor	0.1871
Rwork	0.186
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lu9
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.500	1.500
Number of reflections	1110526	191566
<I/σ(I)>	9.6
Completeness [%]	95.5
Redundancy	3.9
CC(1/2)	0.999	0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	22% PEG4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate