6TG8
Crystal structure of the Kelch domain in complex with 11 amino acid peptide (model of the ETGE loop)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.296, 75.296, 127.539 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.000 - 2.750 |
| Rwork | 0.181 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zgk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.499 |
| Data reduction software | CrysalisPro (1.171.40.53) |
| Data scaling software | Aimless (7.0.077) |
| Phasing software | AMoRE (7.0.077) |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.208 | 2.800 |
| High resolution limit [Å] | 2.450 | 2.700 |
| Rmerge | 0.110 | 0.614 |
| Number of reflections | 11948 | 1198 |
| <I/σ(I)> | 40.66 | |
| Completeness [%] | 99.5 | |
| Redundancy | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.8 M sodium acetate pH 7.0, 0.1 M Bis-Tris propane pH 7.0 |
