6TD4
IRF4 DNA-binding domain surface entropy mutant apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.874, 67.457, 69.884 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.280 - 1.710 |
| R-factor | 0.2182 |
| Rwork | 0.217 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | personal communication |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.591 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.280 | 1.780 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmeas | 0.041 | 1.120 |
| Number of reflections | 17568 | 2774 |
| <I/σ(I)> | 16.9 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 5.7 |
| CC(1/2) | 0.994 | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25 % PEG 3350, 0.1 M HEPES pH 7.5 |
