6TBZ
Crystal structure of the MH1 domain of Smad5-Smad3 chimera construct bound to the GGCGC site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 32 |
| Unit cell lengths | 53.145, 53.145, 83.154 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.000 - 1.782 |
| R-factor | 0.1932 |
| Rwork | 0.192 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fzs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.080 |
| High resolution limit [Å] | 1.780 | 1.820 |
| Rmerge | 0.077 | 0.765 |
| Rmeas | 0.087 | 0.885 |
| Rpim | 0.038 | |
| Number of reflections | 11400 | 571 |
| <I/σ(I)> | 10.9 | 1.9 |
| Completeness [%] | 88.8 | 68.2 |
| Redundancy | 5 | 3.9 |
| CC(1/2) | 0.999 | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 22% PEG 3350, 0.05 M sodium citrate pH 5.5 |
