6T6J
Crystal Structure of the C-terminal domain of the HIV-1 Integrase (subtype A2, mutant N254K, K340Q)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-24 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.306, 48.835, 66.749 |
Unit cell angles | 90.00, 110.37, 90.00 |
Refinement procedure
Resolution | 41.679 - 2.003 |
R-factor | 0.1716 |
Rwork | 0.166 |
R-free | 0.22030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6t6i |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3311) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.679 | 2.080 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 17471 | 1697 |
<I/σ(I)> | 15.15 | |
Completeness [%] | 99.7 | |
Redundancy | 6.5 | |
CC(1/2) | 0.998 | 0.942 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 1M sodium malonate |