6T58

Structure determination of the transactivation domain of p53 in complex with S100A4 using annexin A2 as a crystallization chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	95.960, 62.770, 106.020
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	47.980 - 3.100
R-factor	0.2336
Rwork	0.230
R-free	0.27210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xjl 3zwh
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.980	47.980	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.157	0.029	0.912
Rmeas	0.170	0.031	1.010
Total number of observations	155290
Number of reflections	23244	285	1699
<I/σ(I)>	10	52.03	1.74
Completeness [%]	99.8	96.6	99.9
Redundancy	6.681	7.042	5.377
CC(1/2)	0.995	0.999	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.12 M Alcohols 0.1 M Sodium HEPES; MOPS (acid) pH7.5 20% v/v Ethylene glycol; 10 % w/v PEG 8000 Morpheus D6