6T2P

Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9159
Spacegroup name	P 41
Unit cell lengths	82.724, 82.724, 121.682
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.720 - 2.100
Rwork	0.186
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iln
RMSD bond length	0.010
RMSD bond angle	1.589
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.720	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	47588	3811
<I/σ(I)>	8.7	1.6
Completeness [%]	99.7
Redundancy	6.8
CC(1/2)	0.996	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	450 mM sodium malonate pH 7.0, 24% PEG3350