6T21
N-terminal domain of EcoKMcrA restriction endonuclease (NEco) in complex with T5mCGA target sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 112.882, 112.882, 155.751 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.380 - 2.070 |
R-factor | 0.161 |
Rwork | 0.159 |
R-free | 0.20130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6r64 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.152 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.380 | 2.190 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.103 | 2.299 |
Rmeas | 0.105 | 2.329 |
Number of reflections | 36261 | 5681 |
<I/σ(I)> | 30.17 | 1.98 |
Completeness [%] | 99.5 | 98.6 |
Redundancy | 38.87 | 38.92 |
CC(1/2) | 1.000 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.8 ul of protein-DNA solution (436:523 uM) was mixed with 2.2 ul of the F1 condition of the PACT premier crystal screen (MDL) (0.2 M Sodium fluoride, 0.1 M Bis-Tris propane, pH 6.5, 20% PEG 3350) and cryo-protected with an addition of 25% glycerol. |