6T1Z

LmrP from L. lactis, in an outward-open conformation, bound to Hoechst 33342

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97166
Spacegroup name	C 2 2 21
Unit cell lengths	104.760, 139.990, 115.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.225
Rwork	0.225
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	In-house structure
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.875	2.980
High resolution limit [Å]	2.900	2.900
Number of reflections	12755	281
<I/σ(I)>	13.36
Completeness [%]	66.4
Redundancy	21
CC(1/2)	1.000	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	80 mM SPG pH 7, 20% (w/v) PEG 1500