6SWU

Crystal structure of the TPR domain of KLC1 in complex with an engineered high-affinity cargo peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	101.732, 107.532, 222.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	111.325 - 2.849
Rwork	0.202
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nf1 6fv0
RMSD bond length	0.008
RMSD bond angle	1.421
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.325	2.900
High resolution limit [Å]	2.849	2.850
Rmerge	0.114	1.543
Rmeas	0.131	1.791
Rpim	0.063	0.889
Number of reflections	57529	2758
<I/σ(I)>	8	1.2
Completeness [%]	99.3	97.1
Redundancy	4.47	4.42
CC(1/2)	0.994	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	300	0.1 M MIB buffer pH 9.0, 25% PEG 1500