6SVM

Crystal structure of human GFAT-1 in complex with Glucose-6-Phosphate, L-Glu, and UDP-GalNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	151.995, 151.995, 165.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.150 - 2.481
R-factor	0.1896
Rwork	0.189
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r4e
RMSD bond length	0.003
RMSD bond angle	0.724
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHENIX (dev_2499)
Refinement software	PHENIX (dev_2499)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.160	2.570
High resolution limit [Å]	2.481	2.481
Number of reflections	68982	6701
<I/σ(I)>	14.75
Completeness [%]	99.8
Redundancy	8.7
CC(1/2)	0.999	0.496

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 8.75, 0.2 M Potassium sodium tartrate, 20 % PEG3350