6SVL
human Myeloid-derived growth factor (MYDGF) in complex with neutralizing Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.999890 |
Spacegroup name | P 1 |
Unit cell lengths | 89.519, 107.430, 109.369 |
Unit cell angles | 71.54, 86.25, 73.38 |
Refinement procedure
Resolution | 97.820 - 1.580 |
R-factor | 0.182 |
Rwork | 0.181 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iqw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.820 | 1.584 |
High resolution limit [Å] | 1.580 | 1.584 |
Rmerge | 0.052 | 0.633 |
Number of reflections | 320264 | 160008 |
<I/σ(I)> | 10.2 | 1.8 |
Completeness [%] | 63.5 | |
Redundancy | 2.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 277.15 | 0.1 M trisodium citrate (pH 5.5) and 20 % PEG 3000 |