6SV2
Human prion protein (PrP) fragment 119-231 (G127V M129 variant) complexed to ICSM 18 (anti-Prp therapeutic antibody) Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 300 |
Detector technology | PIXEL |
Collection date | 2013-02-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 128.076, 128.076, 135.642 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 57.930 - 2.300 |
R-factor | 0.2026 |
Rwork | 0.200 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w9e |
RMSD bond length | 0.015 |
RMSD bond angle | 1.735 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.930 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.103 | 1.224 |
Number of reflections | 26908 | 2626 |
<I/σ(I)> | 24.5 | 2.7 |
Completeness [%] | 88.5 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.8 M and 1.5 M ammonium sulphate, 0.1 M Tris (pH 7.5 and 8.0) |