6SUR

The Rab33B-Atg16L1 crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	200
Detector technology	PIXEL
Collection date	2016-05-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	48.400, 204.900, 107.200
Unit cell angles	90.00, 92.60, 90.00

Refinement procedure

Resolution	48.350 - 3.467
R-factor	0.2073
Rwork	0.206
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z06
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.350	48.350	3.600
High resolution limit [Å]	3.467	47.100	3.470
Rmerge	0.194	0.058	0.750
Rmeas	0.229	0.081	0.892
Number of reflections	25983	3	2227
<I/σ(I)>	7.86	14.28	2.2
Completeness [%]	96.3	27.3	79.9
Redundancy	3.466	1.667	3.282
CC(1/2)	0.983		0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES monohydrate pH 6.5, 0.2 M sodium chloride, 10 % (w/v) PEG 4 000 0.1 M spermine