6SQR

Crystal structure of Cat MDM2-S429E RING domain bound to UbcH5B-Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.916
Spacegroup name	P 1 21 1
Unit cell lengths	56.510, 163.880, 70.600
Unit cell angles	90.00, 96.03, 90.00

Refinement procedure

Resolution	70.000 - 2.180
Rwork	0.166
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mnj
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.220
High resolution limit [Å]	2.180	2.180
Number of reflections	65509	3248
<I/σ(I)>	9.4
Completeness [%]	98.8
Redundancy	3.4
CC(1/2)	0.994	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.1 M Tris-HCl, pH 8.5, 20% (v/v) PEG Smear High