6SQR
Crystal structure of Cat MDM2-S429E RING domain bound to UbcH5B-Ub
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | E3 ubiquitin-protein ligase Mdm2 | polymer | 69 | 7849.4 | 1 | UniProt (Q7YRZ8) Pfam (PF13920) | Felis catus (Cat) | Double minute 2 protein,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2 |
| 2 | B, E, H, K (B, E, H, K) | Ubiquitin-conjugating enzyme E2 D2 | polymer | 146 | 16720.2 | 4 | UniProt (P62837) Pfam (PF00179) | Homo sapiens (Human) | (E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1 |
| 3 | C, F, L (C, F, L) | Ubiquitin-40S ribosomal protein S27a | polymer | 77 | 8663.9 | 3 | UniProt (J3QTR3) Pfam (PF00240) UniProt (by SIFTS) (P62979) | Homo sapiens (Human) | |
| 4 | D, J (D, J) | E3 ubiquitin-protein ligase Mdm2 | polymer | 64 | 7249.8 | 2 | UniProt (Q7YRZ8) Pfam (PF13920) | Felis catus (Cat) | Double minute 2 protein,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2 |
| 5 | G (G) | E3 ubiquitin-protein ligase Mdm2 | polymer | 63 | 7152.7 | 1 | UniProt (Q7YRZ8) Pfam (PF13920) | Felis catus (Cat) | Double minute 2 protein,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2 |
| 6 | I (I) | Polyubiquitin-C | polymer | 76 | 8576.8 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (Human) | |
| 7 | AA, CA, DA, GA, HA... (I, J, K, A, B...) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 12 | Chemie (EDO) | |||
| 8 | EA, FA, N, O, T... (J, A, D, G) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 9 | BA, S, Z (I, D, H) | NITRATE ION | non-polymer | 62.0 | 3 | Chemie (NO3) | |||
| 10 | JA, KA, LA, MA, NA... (A, B, C, D, E...) | water | water | 18.0 | 420 | Chemie (HOH) |
Sequence modifications
A: 423 - 491 (UniProt: Q7YRZ8)
B, E, H, K: 2 - 147 (UniProt: P62837)
C, F, L: 1 - 76 (UniProt: J3QTR3)
D, J: 428 - 491 (UniProt: Q7YRZ8)
G: 429 - 491 (UniProt: Q7YRZ8)
| PDB | External Database | Details |
|---|---|---|
| Glu 429 | Ser 429 | engineered mutation |
| Thr 443 | Gly 443 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 22 | Ser 22 | engineered mutation |
| Lys 85 | Cys 85 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 429 | Ser 429 | engineered mutation |
| Thr 443 | Gly 443 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Glu 429 | Ser 429 | engineered mutation |
| Thr 443 | Gly 443 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 130951.1 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 1454.1 | |
| All* | Total formula weight | 132405.2 |
