6SQO

Crystal structure of human MDM2 RING domain homodimer bound to UbcH5B-Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.916
Spacegroup name	P 61
Unit cell lengths	129.709, 129.709, 70.537
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	112.000 - 1.410
Rwork	0.139
R-free	0.17300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mnj
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.000	1.430
High resolution limit [Å]	1.410	1.410
Rmerge	0.047
Number of reflections	129794	6423
<I/σ(I)>	19.2
Completeness [%]	100.0
Redundancy	8.9
CC(1/2)	1.000	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.1 M SPG, pH 7.0, 10% (w/v) PEG Smear Broad, 0.15 M NH4NO3