6SQO
Crystal structure of human MDM2 RING domain homodimer bound to UbcH5B-Ub
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, D | E3 ubiquitin-protein ligase Mdm2 | polymer | 62 | 6920.5 | 2 | UniProt (Q00987) Pfam (PF13920) In PDB | Homo sapiens (Human) | Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2 |
2 | B, E | Ubiquitin-conjugating enzyme E2 D2 | polymer | 146 | 16720.2 | 2 | UniProt (P62837) Pfam (PF00179) In PDB | Homo sapiens (Human) | (E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1 |
3 | C, F | Ubiquitin-40S ribosomal protein S27a | polymer | 77 | 8663.9 | 2 | UniProt (J3QTR3) Pfam (PF00240) UniProt (by SIFTS) (P62979) In PDB | Homo sapiens (Human) | |
4 | A, D | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
5 | A, B, D, E | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
6 | B, E | NITRATE ION | non-polymer | 62.0 | 2 | Chemie (NO3) | |||
7 | water | water | 18.0 | 546 | Chemie (HOH) |
Sequence modifications
B, E: 2 - 147 (UniProt: P62837)
C, F: 1 - 76 (UniProt: J3QTR3)
PDB | External Database | Details |
---|---|---|
Arg 22 | Ser 22 | engineered mutation |
Lys 85 | Cys 85 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 64609.2 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 527.5 | |
All* | Total formula weight | 65136.7 |