6SQO

Crystal structure of human MDM2 RING domain homodimer bound to UbcH5B-Ub

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	E3 ubiquitin-protein ligase Mdm2	polymer	62	6920.5	2	UniProt (Q00987) Pfam (PF13920)	Homo sapiens (Human)	Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
2	B, E (B, E)	Ubiquitin-conjugating enzyme E2 D2	polymer	146	16720.2	2	UniProt (P62837) Pfam (PF00179)	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
3	C, F (C, F)	Ubiquitin-40S ribosomal protein S27a	polymer	77	8663.9	2	UniProt (J3QTR3) Pfam (PF00240) UniProt (by SIFTS) (P62979)	Homo sapiens (Human)
4	G, H, L, M (A, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	I, J, N, O (A, B, D, E)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
6	K, P (B, E)	NITRATE ION	non-polymer		62.0	2	Chemie (NO3)
7	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	546	Chemie (HOH)

Sequence modifications

B, E: 2 - 147 (UniProt: P62837)

PDB	External Database	Details
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

C, F: 1 - 76 (UniProt: J3QTR3)

PDB	External Database	Details
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	64609.2
Non-Polymers*	Number of molecules	10
	Total formula weight	527.5
All*	Total formula weight	65136.7

*Water molecules are not included.