6SQM
Crystal structure of CREBBP bromodomain complexed with LA36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.800, 37.600, 90.460 |
| Unit cell angles | 90.00, 116.20, 90.00 |
Refinement procedure
| Resolution | 43.200 - 1.800 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mme |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 35327 | 5577 |
| <I/σ(I)> | 15.88 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.37 | |
| CC(1/2) | 0.999 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 8% Ethylene glycol, 0.1M HEPES pH 7.5, 10% PEG 8000 |
