6SQG
Crystal structure of viral rhodopsin OLPVRII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.582, 99.699, 82.958 |
| Unit cell angles | 90.00, 117.17, 90.00 |
Refinement procedure
| Resolution | 41.370 - 1.900 |
| R-factor | 0.1726 |
| Rwork | 0.171 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hyj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.370 | 41.370 | 1.930 |
| High resolution limit [Å] | 1.900 | 10.230 | 1.900 |
| Rmerge | 0.081 | 0.023 | 1.613 |
| Rmeas | 0.092 | 0.027 | 1.822 |
| Rpim | 0.043 | 0.013 | 0.838 |
| Total number of observations | 2432 | 20909 | |
| Number of reflections | 89527 | 593 | 4556 |
| <I/σ(I)> | 10.7 | 42.3 | 1.1 |
| Completeness [%] | 98.9 | 95.9 | 98.7 |
| Redundancy | 4.5 | 4.1 | 4.6 |
| CC(1/2) | 0.999 | 0.999 | 0.382 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.2 M sodium malonate, pH 4.6 and 15% PEG 550 MME |
