6SQC
Crystal structure of complex between nuclear coactivator binding domain of CBP and [1040-1086]ACTR containing alpha-methylated Leu1055 and Leu1076
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.180, 42.460, 113.790 |
| Unit cell angles | 90.00, 101.13, 90.00 |
Refinement procedure
| Resolution | 41.720 - 2.280 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.27410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h7q |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.648 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.720 | 2.362 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 21924 | 2175 |
| <I/σ(I)> | 9.58 | 1.94 |
| Completeness [%] | 97.2 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.995 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG6000, 100 mM Tris pH 8 and 10 mM ZnCl2 |
