6SQ2
Structure of a phosphomimetic switch 2 variant of Rab8a in complex with the phospho-Rab binding domain of RILPL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.698, 71.733, 116.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.067 - 1.684 |
| R-factor | 0.1825 |
| Rwork | 0.181 |
| R-free | 0.20230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lhw |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.070 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.055 | 0.402 |
| Rmeas | 0.060 | 0.520 |
| Rpim | 0.025 | 0.323 |
| Number of reflections | 56711 | 2041 |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 97.3 | 69.9 |
| Redundancy | 5.7 | 2 |
| CC(1/2) | 0.998 | 0.616 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 100mM HEPES 10% PEG 4,000 10% 2-propanol |
