6SQ2

Structure of a phosphomimetic switch 2 variant of Rab8a in complex with the phospho-Rab binding domain of RILPL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	60.698, 71.733, 116.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.067 - 1.684
R-factor	0.1825
Rwork	0.181
R-free	0.20230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lhw
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.070	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.055	0.402
Rmeas	0.060	0.520
Rpim	0.025	0.323
Number of reflections	56711	2041
<I/σ(I)>	20.1
Completeness [%]	97.3	69.9
Redundancy	5.7	2
CC(1/2)	0.998	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	100mM HEPES 10% PEG 4,000 10% 2-propanol