6SPR

Structure of the Escherichia coli methionyl-tRNA synthetase variant VI298 complexed with beta-methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	78.310, 45.110, 86.210
Unit cell angles	90.00, 107.39, 90.00

Refinement procedure

Resolution	48.560 - 1.480
R-factor	0.1329
Rwork	0.131
R-free	0.16780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qqt
RMSD bond length	0.008
RMSD bond angle	0.947
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	1.570
High resolution limit [Å]	1.480	1.480
Number of reflections	96417	15234
<I/σ(I)>	9.2	1.3
Completeness [%]	99.6
Redundancy	6.8
CC(1/2)	0.998	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	1.08 M ammonium citrate, 10 mM potassium phosphate, pH 7.0