6SP3

mouse Interleukin-12 subunit beta - p80 homodimer in space group P21 crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972
Spacegroup name	P 1 21 1
Unit cell lengths	53.800, 176.140, 54.370
Unit cell angles	90.00, 108.96, 90.00

Refinement procedure

Resolution	50.880 - 3.000
R-factor	0.2407
Rwork	0.239
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sff
RMSD bond length	0.003
RMSD bond angle	0.654
Data reduction software	XDS (20170615)
Data scaling software	XDS (20170615)
Phasing software	PHASER (2.8.0)
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.714	58.714	3.180
High resolution limit [Å]	2.990	8.860	2.990
Rmeas	0.078	0.029	1.358
Number of reflections	19059	744	2957
<I/σ(I)>	12.59	43.56	1.11
Completeness [%]	98.9
Redundancy	4.13	3.88	4.16
CC(1/2)	0.999	0.999	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287	5% MPD, 10% PEG6000, 100mM HEPES pH7.5 (Crystal screen HT condition 78)