6SMC
mouse Interleukin-12 subunit beta - p80 homodimer in space group P1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 |
Unit cell lengths | 50.074, 96.791, 107.780 |
Unit cell angles | 68.59, 76.84, 85.83 |
Refinement procedure
Resolution | 48.760 - 3.500 |
R-factor | 0.2818 |
Rwork | 0.279 |
R-free | 0.30980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sff |
RMSD bond length | 0.003 |
RMSD bond angle | 0.682 |
Data reduction software | XDS (20190315) |
Data scaling software | XDS (20190315) |
Phasing software | PHASER (2.8.3) |
Refinement software | BUSTER |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.760 | 48.760 | 3.710 |
High resolution limit [Å] | 3.500 | 10.280 | 3.500 |
Rmeas | 0.233 | 0.058 | 1.852 |
Number of reflections | 21609 | 821 | 3445 |
<I/σ(I)> | 5.49 | 23.46 | 0.77 |
Completeness [%] | 92.9 | 89.6 | 91.9 |
Redundancy | 4.12 | 4.05 | 4.09 |
CC(1/2) | 0.992 | 0.996 | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 287 | 200 MM NAFORMATE, 100 MM NAPO4 PH 6.2, 10 % GLYCEROL, 20% MEDIUM PEG SMEAR (BCS CONDITION 40) |